四、結(jié)論

油和/或 Corexit 的存在會(huì)導(dǎo)致 EPS 的蛋白質(zhì)：多糖比率更高，并在中胚層實(shí)驗(yàn)中降低 SFT。 在這些實(shí)驗(yàn)中，SFT 與 蛋白質(zhì)：具有負(fù)斜率的 EPS 多糖。 當(dāng)開闊的海洋 水域和兩種不同的沿海水處理進(jìn)行了比較， 蛋白質(zhì)趨勢(shì)：多糖為 CEWAF > DCEWAF > WAF ≥ Control 并且對(duì)于 SFT，它是相反的， CEWAF < DCEWAF < WAF ≤ 對(duì)照。 因此，SFT 與膠體 EPS 中的蛋白質(zhì)：多糖比率成反比。

當(dāng)中宇宙水柱的不同尺寸分?jǐn)?shù)為 相比之下，我們發(fā)現(xiàn) EPS 膠體可以降低 SFT 蛋白質(zhì)：多糖比例，表明有效的生物乳化 蛋白質(zhì)的容量。 粒子濾波中 SFT 的比較 分?jǐn)?shù) (< 0.45 μm) 和 EPS 膠體分?jǐn)?shù) (< 0.45 μm 和 > 3 kDa)，對(duì)于真正溶解的部分 (< 3 kDa)，它是 表明只有前兩個(gè)包含 EPS 的部分具有容量 以降低 SFT，而 < 3 kDa 級(jí)分顯示與以下相同的 SFT 純海水或只有真正溶解有機(jī)碳的海水。

顯微鏡技術(shù)（即 CLSM 和 SEM）證實(shí)，正如預(yù)測(cè)的那樣，蛋白質(zhì)主要在空氣 - 水界面富集， 強(qiáng)烈影響空氣/水界面處的 SFT 治療。 這些技術(shù)還可視化了不同的聚集體尺寸 和它們的分散，以及聚集體形成的重要性 通過(guò)陰離子EPS組分部分之間的Ca2+"橋接"。 SFT 可能會(huì)發(fā)生微小的變化，與蛋白質(zhì)：多糖比率的變化相吻合，這可能是 pH 值變化的原因（十分之一） 單位），如 EPS 模型化合物所示，這可能在 CMC 周圍最為突出。 此外，我們表明蛋白質(zhì)和酸性多糖的 EPS 模型成分比 Corexit 導(dǎo)致海水中膠束的自組裝甚至 當(dāng)這些成分的濃度很低時(shí)。 這個(gè) 表明 EPS 在形成方面與 Corexit 相同或更有效 乳液。 然而，關(guān)于相互作用的更系統(tǒng)的研究 不同組件的不同組合，以及更多型號(hào) 單獨(dú)的化合物，可能需要更多地闡明在我們的中宇宙實(shí)驗(yàn)中觀察到的復(fù)雜性。

致謝

這項(xiàng)研究得到了墨西哥灣的資助 支持名為 ADDOMEx 的聯(lián)盟研究的研究計(jì)劃 (微生物對(duì)分散劑和油的聚集和降解 Exopolymers) 聯(lián)盟。 原始數(shù)據(jù)可以在海灣找到 墨西哥研究倡議信息和數(shù)據(jù)合作組織 (GRIIDC) 在網(wǎng)址 https://doi.org/10.7266/N7PK0D64； https://doi.org/10。 7266/N78P5XZD； https://doi.org/10.7266/N74X568X； https://doi. org/10.7266/N79W0D1K。

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蛋白質(zhì)外聚物中多糖的比例——摘要、簡(jiǎn)介

蛋白質(zhì)外聚物中多糖的比例——方法

蛋白質(zhì)外聚物中多糖的比例——結(jié)果與討論

蛋白質(zhì)外聚物中多糖的比例——結(jié)論、致謝！